The game features a single-player mode consisting of ten levels in which the player assumes the role of one of two space marines as they attempt to stop the alien race of TimeSplitters from ruining history by collecting the time crystals in various time periods, ranging from the Wild West to the 25th century. It has several multiplayer modes.
The weapons of TimeSplitters 2 include handguns, rifles, submachine guns, shotguns, rocket launchers, a grenade launcher, various explosives, a crossbow, a flamethrower, a fire extinguisher and a brick. They are of many different time periods, from the historical to the futuristic. Some weapons have an alternate fire which activates a feature such as launching a grenade or detonating a remote mine. It is possible to dual wield some weapons.